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KMID : 1377020160130010013
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Tissue Engineering and Regenerative Medicine
2016 Volume.13 No. 1 p.13 ~ p.20
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Optimization of recombinant human platelet-derived growth factor-BB encapsulated in Poly (lactic-co-glycolic acid) microspheres for applications in wound healing
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Bae Yun-Ju
Cho Chi-Heung
Lee Woo-Jong
Huh Jeung-Soo
Lim Jeong-Ok
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Abstract
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Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An in vitro migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.
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KEYWORD
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Recombinant human platelet-derived growth factor-BB, Poly (lactic-co-glycolic acid) microsphere, Wound healing, Human immortalized keratinocyte cell line, Migration
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